CRYO FIXATION
HPM 100 High Pressure Freezing Machine
  • High pressure liquid nitrogen system

    • Excellent preservation of ultrastructure

    • No intracellular antifreezing agents required

    • No Leidenfrost phenomenon

  • Thermally insulated process chamber

    • Cooling rates up to 100’000K/s at specimen surface

    • Low LN2 consumption

  • Universal, application specific specimen carriers

    • Easy handling

    • Perfect freezing of specimens up to 8mm diameter

  • Express specimen handling system

    • Specimen loading in less than 3sec

    • State of the art design

    • Compact and mobile

    • Suitable for correlative LM/EM

High pressure freezing is the only method which allows freezing of aqueous samples up to 200µm thickness without visible ice crystal damage and without the use of cryo-protectants.

  • JFD 030 Jet Freezing Device

  • Freezing of specimens up to approximately 10µm thickness without visible ice crystal damage using the sandwich technique
  • No need for cryo-protectants
  • Suitable for suspensions and emulsions
  • Facilitates further processing by freeze fracturing / etching, freeze drying, freeze substitution

Liquid propane is driven from opposite directions onto a specimen sandwich which consists of two specially designed copper platelets.

  • Cryogenic Freezing Bath

  • Production of small quantities of liquid propane
  • Low cost method for simple cryo experiments
 

FREEZE-ETCHING
  • BAF 060 Freeze-Etch / Freeze-Fracture System

  • Contamination- and artefact-free preparation
  • Short preparation time with load-lock vacuum transfer
  • Compatible with VCT 100 vacuum cryo transfer system
  • Coating with variable angles and rotary shadowing

The BAF 060 is a high end preparation unit for precise freeze-etching / -fracturing with subsequent high resolution coating, using two independent electron evaporation guns. the safe and easy loading / unloading is performed through a load-lock, compatible with the VCT 100 vacuum cryo transfer system for complete cryo EM preparation.

  • MED 020/GBE Freeze-Etch / Fracture Unit

  • Compact but modular table top system
  • Two chamber principle
  • Compatible with VCT 100 vacuum cryo transfer system
  • Precise cuts with computer controlled microtome
  • High resolution coating with DC-sputter-head or e-gun evaporation

MED 020/GBE in combination with the VCT 100 vacuum cryo transfer system is the ideal solution for cryo preparation. The Freeze-Etch / -Fracture unit is attachable to a standard BAL-TEC high resolution evaporator for best freeze-etch / -fracture results with or without subsequent coating.

SCD 500 with the VCT 100 vacuum cryo transfer system is a very easy to handle solution for cryo preparation. The mirror fracturing and etching technique with subsequent high resolution sputter coating gives best resultes for routinely work.

  • VCT 100 Vacuum Cryo Transfer System

  • Contamination free transfer between preparation and analysis
  • Vibration free
  • Independent preparation and analysis
  • Full functionality of SEM during preparation
  • Full stage operation
  • Fast and simple exchange between cryo and standard SEM operation

The VCT 100 vacuum cryo transfer system combines highest analysis resolution with maximal flexibility for preparation. The vacuum cryo shuttle links the preparation device with the SEM and allows safe and easy transport between the two.

  • MED 020 Freeze Drying Unit

  • Reproducible results through exact temperature control of specimen cold stage
  • Contamination free transfer of specimens through counter flow loading device
  • Protection against contamination based on integrated 'Meissner' trap
  • Subsequent coating through DC sputtering or evaporation

The MED 020 Freeze Drying Unit is an ideal system for precision controlled freeze drying of SEM samples or TEM cryo sections. The modular design of the MED 020 allows retrofitting the device for various applications.